Detection of Crude Protein Content in Animal Feed Using Near-Infrared Spectroscopy

On May 1, 2024, the NY/T 4427-2023 "Application Guidelines for Near-Infrared Spectroscopy Determination of Feed" officially came into effect. This standard provides application guidelines for the determination of technical parameters of feed components such as moisture, crude fat, crude protein, starch, crude fiber content, and digestibility using near-infrared spectroscopy.

Crude protein is one of the commonly used analytical parameters for evaluating the nutritional quality of feed. It refers to the total nitrogenous substances, including true protein and non-protein nitrogen compounds. Near-infrared spectroscopy (NIRS) has become a widely used surface analysis method among production enterprises in recent years. The national standard GB/T 18868-2002 outlines the rapid determination of moisture, crude protein, crude fiber, crude fat, lysine, and methionine in feed using near-infrared spectroscopy.

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Measurement Principle

When near-infrared monochromatic or composite light illuminates a sample, the sample selectively absorbs light at certain frequency bands, producing an absorption spectrum. By utilizing the overtone and combination vibrational information of hydrogen-containing groups such as C-H, N-H, and O-H in organic molecules, specific chemometric methods (such as partial least squares regression) can correlate the near-infrared absorption spectrum with its chemical properties and nutritional content. This establishes a relationship between the spectrum and the parameters to be measured (known as a calibration model or database), allowing for rapid analysis of samples using the model. Once the spectrum of the sample is measured, the required protein content parameter data can be quickly obtained by comparing it to the database.

Advantages

The advantages of using near-infrared spectrometers for measuring feed components include efficiency, speed, accuracy, non-destructiveness, and lower cost. They can simultaneously determine multiple components in the feed within a short time, avoiding the issues of traditional analytical methods that require sample destruction, are time-consuming, and have high costs.

Recommended Equipment

● Jinsp SR50R17: This model has a wavelength range of 900 nm to 1700 nm, covering a broad conventional near-infrared detection range. It uses a non-cooled InGaAs sensor with high sensitivity, and its lenses have high near-infrared reflectance efficiency, enabling the detection of weak absorption signals. Its high resolution allows for better distinction of characteristic peaks. It is compact, facilitating experimental setups and complete integration.

● Jinsp SR100N25: This model features a wavelength range of 900 nm to 2500 nm, utilizing a cooled InGaAs sensor known for high resolution and low stray light. Its lens surface is coated with a gold film for high near-infrared reflectance efficiency. It offers various information acquisition methods for convenient integration.

Both of these Jinsp near-infrared spectrometers can be applied in the quality detection of grains and feeds, as well as the detection of substances such as fats, oils, proteins, and fibers. By using either reflection or transmission detection modes in the near-infrared spectral region, the absorption intensity of the feed samples can be measured, linking the absorbance values with the content or properties of the sample components, thus achieving the goal of detecting feed component content. For more details, please visit:

●  Best SR50R17 Near Infrared Non-cooled Spectrometer manufacturers and suppliers | JINSP

●  Best SR100N17/N25 Cooled Near-Infrared Spectrometer manufacturers and suppliers | JINSP


Post time: Nov-01-2024