Study on Real-Time Detection of Glucose Concentration in Microbial Fermentation Process by Raman Spectroscopy

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Real-time monitoring of key parameters during microbial fermentation processes is crucial in the fields of biomedicine and food fermentation. In recent years, Raman spectroscopy technology has emerged as a research hotspot in this field due to its unique advantages. Particularly for samples with high water content, such as microbial fermentation broth, Raman spectroscopy has demonstrated excellent analytical capabilities.

Recently, a study on the real-time detection of glucose concentration during microbial fermentation processes using Raman spectroscopy has achieved significant results. This study employed the RS2000 portable Raman device from JINSP, integrated with its high-performance transmission spectrometer ST100S, to achieve in-situ, contamination-free, rapid real-time online detection.

Through in-depth analysis, they successfully established a rapid detection method for glucose concentration based on Raman spectroscopy. Using the SO₄^2– (1642 cm^–1) response peak as an internal standard, the representative characteristic peaks were extracted using dimensionality reduction analysis in multivariate analysis. The standard curve was generated by dividing the arithmetic mean of the variable importance in projection (VIP) data from the top 10 variables by the peak height of the internal standard, resulting in a linear correlation coefficient r=0.995, fully demonstrating the accuracy and reliability of the technique

It is worth mentioning that JINSP^® ST100S transmission spectrometer played a crucial role in this study. Its features, such as high diffraction efficiency, excellent imaging, high-performance camera, and high resolution, make it significantly advantageous in both scientific research and industrial applications. This spectrometer employs a Volume Phase Holographic (VPH) grating with a diffraction efficiency of nearly 90%, combined with an optimized optical path design close to the diffraction limit, offering superior imaging results. Additionally, it is equipped with a research-grade cryogenic cooled CCD camera, ensuring high sensitivity and signal-to-noise ratio, supporting multi-channel acquisition with a resolution of up to 3 cm^-1.

In summary, Raman spectroscopy technology has demonstrated great potential and application value in real-time online detection of glucose concentration in microbial fermentation processes. By combining with JINSP^® ST100S transmission spectrometer, rapid, accurate, and real-time online detection can be achieved, providing powerful technical support for the development of biomedicine and food fermentation fields. For more details, please visit:

3 Instrument recommendation |ST100S transmission spectrometer

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